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Proceedings Paper

Advances in zinc sensors for studying zinc release events from pancreatic cells
Author(s): Zeev Rosenzweig; Nitsa Rosenzweig; Georgeta Crivat
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Paper Abstract

This paper describes the fabrication and characterization of the analytical properties of fluorescence-based zinc ion sensing glass slides and antibody based zinc sensors and their application in monitoring zinc release from beta pancreatic cells. The zinc ion indicator ZnAF-2 {6-[N- [N', N'-bis (2-pyridinylmethyl)-2-aminoethyl] amino-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H] xanthene]-3-one} was modified to include a sufficiently long linking aliphatic chain, with a terminal carboxyl functional group. The activated carboxyl-modified ZnAF-2 was conjugated to the amino silanized surface of glass slides and to free amino groups of the A2B5 antibody molecules. The sensors were used to monitor zinc ion release events from glucose-stimulated pancreatic cells.

Paper Details

Date Published: 25 October 2006
PDF: 8 pages
Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 638002 (25 October 2006); doi: 10.1117/12.690653
Show Author Affiliations
Zeev Rosenzweig, Univ. of New Orleans (United States)
Nitsa Rosenzweig, Univ. of New Orleans (United States)
Georgeta Crivat, Univ. of New Orleans (United States)

Published in SPIE Proceedings Vol. 6380:
Smart Medical and Biomedical Sensor Technology IV
Brian M. Cullum; J. Chance Carter, Editor(s)

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