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Proceedings Paper

Fluorescence excitation-emission matrices of selected biological materials
Author(s): M. Wlodarski; M. Kaliszewski; M. Kwasny; K. Kopczynski; Z. Zawadzki; Z. Mierczyk; J. Mlynczak; E. Trafny; M. Szpakowska
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Paper Abstract

EX-EM fluorescence matrices from suspensions of 11 vegetative bacteria, 5 spores, and 14 interferents (fungi, pollens) were measured and cross-sections of the selected bacteria were calculated. The changes of fluorescence characteristics of vegetative cells during their sporulation and starvation to death as well as spores during germination are shown. Influence of culture media on emission spectra and rate of spores formation under starving conditions was examined. Analysis of the measured fluorescence characteristics shows that double- or multi-wavelenghts excitation can make it possible to distinguish between particular groups of biological material, i.e., spores, vegetative cells, proteins, and interferents.

Paper Details

Date Published: 13 October 2006
PDF: 12 pages
Proc. SPIE 6398, Optically Based Biological and Chemical Detection for Defence III, 639806 (13 October 2006); doi: 10.1117/12.687872
Show Author Affiliations
M. Wlodarski, Military Univ. of Technology (Poland)
M. Kaliszewski, Military Univ. of Technology (Poland)
M. Kwasny, Military Univ. of Technology (Poland)
K. Kopczynski, Military Univ. of Technology (Poland)
Z. Zawadzki, Military Univ. of Technology (Poland)
Z. Mierczyk, Military Univ. of Technology (Poland)
J. Mlynczak, Military Univ. of Technology (Poland)
E. Trafny, Military Institute of Hygiene & Epidemiology (Poland)
M. Szpakowska, Military Institute of Hygiene & Epidemiology (Poland)


Published in SPIE Proceedings Vol. 6398:
Optically Based Biological and Chemical Detection for Defence III
John C. Carrano; Arturas Zukauskas, Editor(s)

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