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Proceedings Paper

Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector
Author(s): X. Michalet; O.H. W. Siegmund; J. V. Vallerga; P. Jelinsky; F. Pinaud; J. E. Millaud; S. Weiss
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Paper Abstract

We have recently developed a wide-field photon-counting detector (the H33D detector) having high-temporal and highspatial resolutions and capable of recording up to 500,000 photons per sec. Its temporal performance has been previously characterized using solutions of fluorescent materials with different lifetimes, and its spatial resolution using sub-diffraction objects (beads and quantum dots). Here we show its application to fluorescence lifetime imaging of live cells and compare its performance to a scanning confocal TCSPC approach. With the expected improvements in photocathode sensitivity and increase in detector throughput, this technology appears as a promising alternative to the current lifetime imaging solutions.

Paper Details

Date Published: 25 October 2006
PDF: 8 pages
Proc. SPIE 6372, Advanced Photon Counting Techniques, 63720E (25 October 2006); doi: 10.1117/12.686429
Show Author Affiliations
X. Michalet, Univ. of California, Los Angeles (United States)
O.H. W. Siegmund, Univ. of California, Berkeley (United States)
J. V. Vallerga, Univ. of California, Berkeley (United States)
P. Jelinsky, Univ. of California, Berkeley (United States)
F. Pinaud, Univ. of California/Los Angeles (United States)
J. E. Millaud, Lawrence Livermore National Lab. (United States)
S. Weiss, Univ. of California, Los Angeles (United States)

Published in SPIE Proceedings Vol. 6372:
Advanced Photon Counting Techniques
Wolfgang Becker, Editor(s)

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