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Proceedings Paper

Analysis of oxytetracycline in surface water using a portable analyzer
Author(s): Guoying Chen; Susan Braden; Feng Qin
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Paper Abstract

Oxytetracycline (OTC) is extensively used in aquaculture worldwide for preventive and therapeutic purposes. Most of the drug, however, is discharged into the marine environment due to leaching from medicated feed and poor gastrointestinal (GI) absorption. Without exposure to sun light OTC has a long lifetime in the marine environment, therefore it is important to monitor and study its occurrence, distribution, fate and impact on the ecosystem. A portable tetracycline (TC) analyzer was developed in this laboratory for this purpose based on europium-sensitized luminescence. In this study, an assay method is developed for OTC analysis in water using this instrument. Water samples are filtered with glass wool; and solid phase extraction (SPE) is performed using Oasis HLB cartridges for OTC extraction and cleanup. Following reagent application, the samples are excited by 385 nm LED pulses; and time-resolved luminescence (TRL) is measured at 610 nm by a photomultiplier tube. A 0-3 ppm linear dynamic range (r2 = 0.9988) and a 0.021 ppb limit of detection were achieved with a typical <5% relative standard deviation.

Paper Details

Date Published: 25 October 2006
PDF: 6 pages
Proc. SPIE 6378, Chemical and Biological Sensors for Industrial and Environmental Monitoring II, 63780W (25 October 2006); doi: 10.1117/12.686401
Show Author Affiliations
Guoying Chen, U.S. Dept of Agriculture (United States)
Susan Braden, U.S. Dept of Agriculture (United States)
Feng Qin, Shanghai Institute of Drug Control (China)

Published in SPIE Proceedings Vol. 6378:
Chemical and Biological Sensors for Industrial and Environmental Monitoring II
Steven D. Christesen; Arthur J. Sedlacek III; James B. Gillespie; Kenneth J. Ewing, Editor(s)

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