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Proceedings Paper

Evaluation of aptamers as molecular recognition elements for pathogens using capillary electrophoretic analysis
Author(s): Sun McMasters; Dimitra N. Stratis-Cullum
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Paper Abstract

The biomolecular interactions between a fluorescently labeled aptamer and whole cell Campylobacter jejuni(C. jejuni) have been characterized using capillary electrophoresis with laser-induced fluorescence detection. From electrophoretic analysis, the bound complex forms, unbound aptamer, and cells were visualized. The relative binding affinity of the DNA aptamer with C. jejuni was compared with other food-borne pathogens including Escherichia coli O157:H7 and Salmonella typhimurium. Preliminary data suggests that this aptamer exhibits strong binding affinity towards C. jejuni with minimal cross reactivity over other food pathogens when equivalent cell concentrations were used.

Paper Details

Date Published: 23 October 2006
PDF: 8 pages
Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 63800B (23 October 2006); doi: 10.1117/12.686357
Show Author Affiliations
Sun McMasters, Army Research Lab. (United States)
Dimitra N. Stratis-Cullum, Army Research Lab. (United States)

Published in SPIE Proceedings Vol. 6380:
Smart Medical and Biomedical Sensor Technology IV
Brian M. Cullum; J. Chance Carter, Editor(s)

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