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Proceedings Paper

Raman molecular imaging of tissue and cell samples using tunable multiconjugate filter
Author(s): John Maier; Janice Panza; Amy Drauch; Shona Stewart
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Paper Abstract

Raman spectroscopy is a powerful technique for rapid, non-invasive and reagentless analysis of materials, including biological cells and tissues. Raman Molecular Imaging combines high molecular information content Raman spectroscopy and digital full field imaging to enable the investigation of cells and tissues. We have conducted widefield imaging using a new class of birefringent liquid crystal tunable filter that provides high throughput over an extended wavelength range. This tool has been applied to investigate the linkage between reagentless spectral imaging in tissue and cells and standard reagent based approaches. In this report, we describe Raman imaging data on a clinical tissue sample and cultured cells. The results demonstrate the sensitivity of Raman Molecular Imaging and fluorescence spectral imaging to molecular differences in biological systems laying the foundation for the eventual use of this approach as a biological research and clinical diagnostic tool.

Paper Details

Date Published: 23 October 2006
PDF: 12 pages
Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 638009 (23 October 2006); doi: 10.1117/12.686243
Show Author Affiliations
John Maier, ChemImage Corp. (United States)
Janice Panza, ChemImage Corp. (United States)
Amy Drauch, ChemImage Corp. (United States)
Shona Stewart, ChemImage Corp. (United States)

Published in SPIE Proceedings Vol. 6380:
Smart Medical and Biomedical Sensor Technology IV
Brian M. Cullum; J. Chance Carter, Editor(s)

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