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Proceedings Paper

Imaging technology of three dimensional distribution for sugar chain on single living cell membrane
Author(s): Kazuya Yamamoto; Ichirou Ishimaru; Yoshiki Fujii; Toshiki Yasokawa; Katsumi Ishizaki; Makoto Yoshida; Kaoru Takegawa; Naotaka Tanaka; Shigeki Kuriyama; Tsutomu Masaki; Seiji Nakai
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Paper Abstract

We study on the imaging technology of three-dimensional distribution for sugar chain on single living cell-membrane. This technology can observe the entire cell surface. To observe the cell surface, the local area image of cell-membrane is taken by TIRF (total internal reflection fluorescence) microscopy. And by scanning the whole cell surface area, we can obtain the image of the entire cell membrane. These observed local area images can be converted into an entire surface image by the pattern matching processing. For this scanning technology, we propose the proximal two beam optical tweezers to rotate the single floating cell. This proximal two beam optical tweezers can rotate the floating single cell in the nutrient medium by light pressure. Two beams illuminate the single cell at proximal two points from below and above. The cell is trapped at the center of these two focal points. At the same time, light pressures that are generated at two focal points are made to act as rotational torque. Conventionally TIRF microscope is well known as the observation technology for the cell-membrane using the evanescent light as the exciting light. We can observe the local area images of the fluorescently labeled sugar chain that binds the glycoprotein. Using the proposed optical system, we can obtain the fluorescent distribution images on the cell-membrane.

Paper Details

Date Published: 19 October 2006
PDF: 9 pages
Proc. SPIE 6374, Optomechatronic Actuators, Manipulation, and Systems Control, 63740L (19 October 2006); doi: 10.1117/12.685893
Show Author Affiliations
Kazuya Yamamoto, Kagawa Univ. (Japan)
Ichirou Ishimaru, Kagawa Univ. (Japan)
Yoshiki Fujii, Kagawa Univ. (Japan)
Toshiki Yasokawa, Kagawa Univ. (Japan)
Katsumi Ishizaki, Kagawa Univ. (Japan)
Makoto Yoshida, Kagawa Univ. (Japan)
Kaoru Takegawa, Kagawa Univ. (Japan)
Naotaka Tanaka, Kagawa Univ. (Japan)
Shigeki Kuriyama, Kagawa Univ. (Japan)
Tsutomu Masaki, Kagawa Univ. (Japan)
Seiji Nakai, Kagawa Univ. (Japan)

Published in SPIE Proceedings Vol. 6374:
Optomechatronic Actuators, Manipulation, and Systems Control
Farrokh Janabi-Sharifi; Yukitoshi Otani, Editor(s)

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