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Proceedings Paper

Development of structured illumination based endoscope
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Paper Abstract

Confocal microscopy is a well established method in optical sectioning. It allows for increased axial resolution. However, it has a longstanding problem in the duration of scanning time and often yield poor contrast in images. Structured illumination is wide-field microscopy technique which delivers optical sectioning capability yet with an improved contrast. Comparing with confocal microscopy, structured illumination required only capturing of 3 frame images to obtain both an optical sectioned image and a conventional image, thus allowing for multiple display style. With these advantages, structured illumination presents excellent opportunities for its usage on non-invasive imaging. This study on structured illumination is done as a part of an effort to develop an endoscopy system. The fringe projection system is based upon light reflected off from TI-DMD digital micromirror and is projected onto the specimen (Allium cepa, onion) via 10×/0.25 NA lens. The superimposed image is then captured with a Pixelink PL-741 with a frame rate of 30 frames/seconds. The 3 captured images are then processed by a computing unit which removed the imposed fringe pattern and recover both the sectioned and conventional image. The study has shown appreciable sectioning capabilities in structured illumination and the fast scan rates demonstrates its feasibility for endoscopy development. Usage of DMD also allows for variable sectioning strength which would prove useful in biological setting. The study finally discusses some common fringe residual problem and their removal method.

Paper Details

Date Published:
PDF: 9 pages
Proc. SPIE 6380, Smart Medical and Biomedical Sensor Technology IV, 63800Y; doi: 10.1117/12.685521
Show Author Affiliations
C. H. Wong, National Univ. of Singapore (Singapore)
N. G. Chen, National Univ. of Singapore (Singapore)
C. J. R. Sheppard, National Univ. of Singapore (Singapore)

Published in SPIE Proceedings Vol. 6380:
Smart Medical and Biomedical Sensor Technology IV
Brian M. Cullum; J. Chance Carter, Editor(s)

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