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Proceedings Paper

Multiwavelength fluorescence lifetime imaging by TCSPC
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Paper Abstract

We present an approach which monitors both time- and spectral information of the fluorescence in order to receive the full information content of the light emitted from a sample. Our instrumentation covers a combination of the TCSPC technique extended by a multi-wavelength detection scheme. Based on the spectral properties of the participating chromophores their relative contributions to the fluorescence within each wavelength channel can be derived. This information serves as an additional parameter for the decay curve analysis which allows us to identify emission patterns for both lifetime and wavelength. Potential applications of the multi-wavelength TCSPC technique are FRET experiments, new marker techniques based on environment-dependent lifetime, and the separation of fluorophores in autofluorescence images of tissue.

Paper Details

Date Published: 25 October 2006
PDF: 6 pages
Proc. SPIE 6372, Advanced Photon Counting Techniques, 637204 (25 October 2006); doi: 10.1117/12.684743
Show Author Affiliations
Axel Bergmann, Becker & Hickl GmbH (Germany)
Wolfgang Becker, Becker & Hickl GmbH (Germany)

Published in SPIE Proceedings Vol. 6372:
Advanced Photon Counting Techniques
Wolfgang Becker, Editor(s)

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