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Proceedings Paper

Imaging tissue structures: assessment of absorption and phase-contrast x-ray tomography imaging at 2nd and 3rd generation synchrotrons
Author(s): Kyungmin Ham; Heath A. Barnett; Tolulope Ogunbakin; Dominique G. Homberger; Hermann H. Bragulla; Kenneth L. Matthews; Clinton S. Willson; Leslie G. Butler
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Paper Abstract

Several methods have been proposed for imaging biological tissue structures at the near micron scale and with user-control of contrast mechanisms that differentiate among the tissue structures. On the one hand, treatment with high-Z contrast agents (Ba, Cs, I, etc.) by injection or soaking and absorption edge imaging distinguishes soft tissue from cornified or bony tissue. This experiment is most compatible with high-bandpass monochromators (ΔE/E between 0.01 - 0.03), such as recently installed at the LSU synchrotron (CAMD). On the other hand, phase contrast imaging does not require any pre-treatment except preservation in formalin, but places more demands upon the X-ray source. This experiment is more compatible with beam lines, such as 13 BM-D at APS, which operates with a narrow bandpass monochromator (ΔE/E ≈ 10-4). Here, we compare imaging results of soft, cornified and bony tissues across the 2x2 matrix of absorption edge versus phase contrast, and high versus narrow bandpass monochromators. In addition, we comment on new data acquisition strategies adapted to the fragile character of biological tissues: (a) a 100 % humidity chamber, and (b) a data acquisition strategy, based on the Greek golden ratio, that more quickly leads to image convergence. The latter incurs the minor cost of reprogramming, or relabeling, images with order and angle. Subsequently, tomography data sets can be acquired based on synchrotron performance and sample fragility.

Paper Details

Date Published: 7 September 2006
PDF: 10 pages
Proc. SPIE 6318, Developments in X-Ray Tomography V, 631822 (7 September 2006); doi: 10.1117/12.681202
Show Author Affiliations
Kyungmin Ham, Louisiana State Univ. (United States)
Heath A. Barnett, Louisiana State Univ. (United States)
Tolulope Ogunbakin, Louisiana State Univ. (United States)
Dominique G. Homberger, Louisiana State Univ. (United States)
Hermann H. Bragulla, Louisiana State Univ. (United States)
Kenneth L. Matthews, Louisiana State Univ. (United States)
Clinton S. Willson, Louisiana State Univ. (United States)
Leslie G. Butler, Louisiana State Univ. (United States)

Published in SPIE Proceedings Vol. 6318:
Developments in X-Ray Tomography V
Ulrich Bonse, Editor(s)

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