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Proceedings Paper

Chemotaxis study using optical tweezers to observe the strength and directionality of forces of Leishmania amazonensis
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Paper Abstract

The displacements of a dielectric microspheres trapped by an optical tweezers (OT) can be used as a force transducer for mechanical measurements in life sciences. This system can measure forces on the 50 femto Newtons to 200 pico Newtons range, of the same order of magnitude of a typical forces induced by flagellar motion. The process in which living microorganisms search for food and run away from poison chemicals is known is chemotaxy. Optical tweezers can be used to obtain a better understanding of chemotaxy by observing the force response of the microorganism when placed in a gradient of attractors and or repelling chemicals. This report shows such observations for the protozoa Leishmania amazomenzis, responsible for the leishmaniasis, a serious tropical disease. We used a quadrant detector to monitor the movement of the protozoa for different chemicals gradient. This way we have been able to observe both the force strength and its directionality. The characterization of the chemotaxis of these parasites can help to understand the infection mechanics and improve the diagnosis and the treatments employed for this disease.

Paper Details

Date Published: 11 September 2006
PDF: 6 pages
Proc. SPIE 6326, Optical Trapping and Optical Micromanipulation III, 63262A (11 September 2006); doi: 10.1117/12.680999
Show Author Affiliations
Liliana de Ysasa Pozzo, UNICAMP (Brazil)
Adriana Fontes, UNICAMP (Brazil)
André A. de Thomaz, UNICAMP (Brazil)
Luiz Carlos Barbosa, UNICAMP (Brazil)
Diana Copi Ayres, UNICAMP (Brazil)
Selma Giorgio, UNICAMP (Brazil)
Carlos Lenz Cesar, UNICAMP (Brazil)


Published in SPIE Proceedings Vol. 6326:
Optical Trapping and Optical Micromanipulation III
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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