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Proceedings Paper

Wave front engineering by means of diffractive optical elements for applications in microscopy
Author(s): Dan Cojoc; Enrico Ferrari; Valeria Garbin; Stefano Cabrini; Alessandro Carpentiero; Mauro Prasciolu; Luca Businaro; Burchard Kaulich; Enzo Di Fabrizio
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Paper Abstract

We present a unified view regarding the use of diffractive optical elements (DOEs) for microscopy applications a wide range of electromagnetic spectrum. The unified treatment is realized through the design and fabrication of DOE through which wave front beam shaping is obtained. In particular we show applications ranging from micromanipulation using optical tweezers to X-ray differential interference contrast (DIC) microscopy. We report some details on the design and physical implementation of diffractive elements that beside focusing perform also other optical functions: beam splitting, beam intensity and phase redistribution or mode conversion. Laser beam splitting is used for multiple trapping and independent manipulation of spherical micro beads and for direct trapping and manipulation of biological cells with non-spherical shapes. Another application is the Gauss to Laguerre-Gaussian mode conversion, which allows to trap and transfer orbital angular momentum of light to micro particles with high refractive index and to trap and manipulate low index particles. These experiments are performed in an inverted optical microscope coupled with an infrared laser beam and a spatial light modulator for DOEs implementation. High resolution optics, fabricated by means of e-beam lithography, are demonstrated to control the intensity and the phase of the sheared beams in X-ray DIC microscopy. DIC experiments with phase objects reveal a dramatic increase in image contrast compared to bright-field X-ray microscopy.

Paper Details

Date Published: 14 June 2006
PDF: 10 pages
Proc. SPIE 6254, Seventh International Conference on Correlation Optics, 625416 (14 June 2006); doi: 10.1117/12.679941
Show Author Affiliations
Dan Cojoc, CNR-INFM, Lab. Nazionale TASC, LILIT Group (Italy)
Politehnica Univ. of Bucharest (Romania)
Enrico Ferrari, CNR-INFM, Lab. Nazionale TASC, LILIT Group (Italy)
Univ. of Trieste (Italy)
Valeria Garbin, CNR-INFM, Lab. Nazionale TASC, LILIT Group (Italy)
Univ. of Trieste (Italy)
Stefano Cabrini, CNR-INFM, Lab. Nazionale TASC, LILIT Group (Italy)
Alessandro Carpentiero, CNR-INFM, Lab. Nazionale TASC, LILIT Group (Italy)
Mauro Prasciolu, CNR-INFM, Lab. Nazionale TASC, LILIT Group (Italy)
Luca Businaro, CNR-INFM, Lab. Nazionale TASC, LILIT Group (Italy)
Burchard Kaulich, ELETTRA (Italy)
Enzo Di Fabrizio, CNR-INFM, Lab. Nazionale TASC, LILIT Group (Italy)
Univ. of Magna Graecia (Italy)


Published in SPIE Proceedings Vol. 6254:
Seventh International Conference on Correlation Optics
Oleg V. Angelsky, Editor(s)

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