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Proceedings Paper

Optical trapping dynamics for cell identification
Author(s): Giovanni Volpe; Gajendra P. Singh; Florian Buettner; Anna M. Drets; Dmitri V. Petrov
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Paper Abstract

Living cells show a variety of morphological traits upon which numerous identification techniques have already been developed. However most of them involve lengthy biochemical procedures and can compromise the viability of the cell. We demonstrate a method to differentiate cells only on the basis of its trapping dynamics while it is being drawn into an optical trap (Optical Trapping Dynamics). Since it relies only on the inherent properties of the optical trap, without requiring external markers or biochemically sensitive spectroscopic techniques, it can be readily combined with existing optical tweezers setups. We applied it to the study of the yeast cell-cycle stages, showing, in particular, how it can be amenable for the measurement of the budding index of a cell population.

Paper Details

Date Published: 30 August 2006
PDF: 9 pages
Proc. SPIE 6326, Optical Trapping and Optical Micromanipulation III, 63260P (30 August 2006); doi: 10.1117/12.679374
Show Author Affiliations
Giovanni Volpe, ICFO, Institut de Ciencies Fotoniques (Spain)
Gajendra P. Singh, ICFO, Institut de Ciencies Fotoniques (Spain)
Florian Buettner, ICFO, Institut de Ciencies Fotoniques (Spain)
Anna M. Drets, ICFO, Institut de Ciencies Fotoniques (Spain)
Dmitri V. Petrov, ICFO, Institut de Ciencies Fotoniques (Spain)
ICREA, Insitucio Catalana de Recerca i Estudis Avancats (Spain)


Published in SPIE Proceedings Vol. 6326:
Optical Trapping and Optical Micromanipulation III
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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