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Proceedings Paper

The interaction of lipopolysaccharide-coated polystyrene particle with membrane receptor proteins on macrophage measured by optical tweezers
Author(s): Ming-Tzo Wei; Kuo-Feng Hua; Jowey Hsu; Artashes Karmenyan; Hsien-Yeh Hsu; Arthur Chiou
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Paper Abstract

Lipopolysaccharide (LPS) is one of the cell wall components of Gram-positive bacteria recognized by and interacted with receptor proteins such as CD14 on macrophage cells. Such a process plays an important role in our innate immune system. In this paper, we report the application of optical tweezers (λ = 1064nm Gaussian beam focused by a water-immersed objective lens with N.A. = 1.0) to the study of the dynamics of the binding of a LPS-coated polystyrene particle (diameter = 1.5μm) onto the plasma membrane of a macrophage cell. We demonstrated that the binding rate increased significantly when the macrophage cell was pre-treated with the extract of Reishi polysaccharides (EORP) which has been shown to enhance the cell surface expression of CD14 (receptor of LPS) on macrophage cells.

Paper Details

Date Published: 14 September 2006
PDF: 10 pages
Proc. SPIE 6326, Optical Trapping and Optical Micromanipulation III, 63260T (14 September 2006); doi: 10.1117/12.679258
Show Author Affiliations
Ming-Tzo Wei, National Yang-Ming Univ. (Taiwan)
Kuo-Feng Hua, National Yang-Ming Univ. (Taiwan)
Jowey Hsu, National Yang-Ming Univ. (Taiwan)
Artashes Karmenyan, National Yang-Ming Univ. (Taiwan)
Hsien-Yeh Hsu, National Yang-Ming Univ. (Taiwan)
Arthur Chiou, National Yang-Ming Univ. (Taiwan)

Published in SPIE Proceedings Vol. 6326:
Optical Trapping and Optical Micromanipulation III
Kishan Dholakia; Gabriel C. Spalding, Editor(s)

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