Share Email Print
cover

Proceedings Paper

A compact optofluidic microscope
Author(s): Xin Heng; Xiquan Cui; David Erickson; Larry R. Baugh; Paul W. Sternberg; Demetri Psaltis; Changhuei Yang
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

We demonstrate a novel optical imaging device that can be directly integrated into a microfluidic network, and therefore enables on-chip imaging in a microfluidic system. This micro imaging device, termed optofluidic microscope (OFM) is free of bulk optics and is based on a nanohole array defined in a non-transmissive metallic layer that is patterned onto the floor of the microfluidic channel. The operation of the optofluidic microscope will be explained in details and its performance is examined by using a popular animal model, Caenorhabditis elegans (C. elegans). Images from a large population of nematode worms are efficiently acquired within a short time frame. The quality of the OFM images of C. elegans and the morphological characteristics revealed therein are evaluated. Two groups of early-stage C. elegans larvae, wild-type and dpy-24 are successfully separated even though their morphological difference at the larval stage is subtle. The experimental results support our claim that the methodology described therein can be effectively used to develop a powerful tool for fulfilling high-resolution, high-throughput imaging task in microfluidics-based systems.

Paper Details

Date Published: 13 September 2006
PDF: 9 pages
Proc. SPIE 6329, Optofluidics, 632908 (13 September 2006); doi: 10.1117/12.678940
Show Author Affiliations
Xin Heng, California Institute of Technology (United States)
Xiquan Cui, California Institute of Technology (United States)
David Erickson, Cornell Univ. (United States)
Larry R. Baugh, California Institute of Technology (United States)
Paul W. Sternberg, California Institute of Technology (United States)
Demetri Psaltis, California Institute of Technology (United States)
Changhuei Yang, California Institute of Technology (United States)


Published in SPIE Proceedings Vol. 6329:
Optofluidics
Demetri Psaltis; Yeshaiahu Fainman, Editor(s)

© SPIE. Terms of Use
Back to Top