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Proceedings Paper

Study of interference laser field action on erythrocyte membranes by fluorescent probes with intramolecular proton transfer
Author(s): Julia V. Kruchenok; Andrei N. Sobchuk; Grigory I. Kurilo; Nicolai A. Nemkovich; Anatoly N. Rubinov
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Paper Abstract

We have investigated structural changes of human erythrocyte membranes under the action of interference light fields with different periods of spatial modulation. A local polarity in the surface regions of membranes was detected by means of the fluorescent probe method using stationary and time-resolved fluorescence spectroscopy. The electro-dipolar molecules 4'-diethylamino (FET) and 4'-N-(15-azacrown-5) (FCR) derivatives of 3-hydroxyflavone with coupled intramolecular charge and proton transfers were used as fluorescent probes. The FET molecules locate deeper in the membrane than FCR probes. It was established that the micropolarity of probes binding sites in membranes is changed after irradiation by the interference laser field. The largest effect was observed when erythrocytes were irradiated by the field with 7.2 μm period, which corresponds to maximal energy of interaction of an erythrocyte with a gradient light field.

Paper Details

Date Published: 19 May 2006
PDF: 7 pages
Proc. SPIE 6257, ICONO 2005: Nonlinear Laser Spectroscopy, High Precision Measurements, and Laser Biomedicine and Chemistry, 62570W (19 May 2006); doi: 10.1117/12.678390
Show Author Affiliations
Julia V. Kruchenok, B.I. Stepanov Institute of Physics (Belarus)
Andrei N. Sobchuk, B.I. Stepanov Institute of Physics (Belarus)
Grigory I. Kurilo, B.I. Stepanov Institute of Physics (Belarus)
Nicolai A. Nemkovich, B.I. Stepanov Institute of Physics (Belarus)
Anatoly N. Rubinov, B.I. Stepanov Institute of Physics (Belarus)


Published in SPIE Proceedings Vol. 6257:
ICONO 2005: Nonlinear Laser Spectroscopy, High Precision Measurements, and Laser Biomedicine and Chemistry
Sergey N. Bagayev; Andrey Chikishev; Alexander Dmitriev; Martial Ducloy; Tony Heinz; Vladilen Letokhov; Alexander Shkurinov; Hiroaki Takahashi, Editor(s)

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