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Proceedings Paper

Optical tracking of micro-objects within living cells
Author(s): J. Ježek; M. Vařecha; J. Amrichova; M. Kozubek; T. Čižmár; P. Zemánek
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Paper Abstract

Tracing of foreign objects inside living cells is very exciting way how to study interior of living objects in nondestructive way. We imported fluorescent submicron particles into the living cells using liposomes as carriers to study the local mechanical heterogeneity of the cell cytoplasm. Thermal motion of these probes within the cell is tracked using fluorescent video-microscopy. The time-records of the probe positions reveal their trajectories and accessible space to the probes inside the cytoplasm of living cells. Further analyses of the thermal motion of the probes can reveal the mechanism of sub-cellular transport and properties of the cytoplasm in vivo.

Paper Details

Date Published: 18 April 2006
PDF: 6 pages
Proc. SPIE 6180, Photonics, Devices, and Systems III, 618025 (18 April 2006); doi: 10.1117/12.675855
Show Author Affiliations
J. Ježek, Institute of Scientific Instruments (Czech Republic)
M. Vařecha, Masaryk Univ. Brno (Czech Republic)
J. Amrichova, Masaryk Univ. Brno (Czech Republic)
M. Kozubek, Masaryk Univ. Brno (Czech Republic)
T. Čižmár, Institute of Scientific Instruments (Czech Republic)
P. Zemánek, Institute of Scientific Instruments (Czech Republic)

Published in SPIE Proceedings Vol. 6180:
Photonics, Devices, and Systems III
Pavel Tománek; Miroslav Hrabovský; Miroslav Miler; Dagmar Senderákova, Editor(s)

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