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Proceedings Paper

SERS and MEF: promising techniques for biomedicine
Author(s): Stefan Kruszewski; Henryk Malak; Blanka Ziomkowska; Michał Cyrankiewicz
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Paper Abstract

SERS and MEF phenomena are the result of resonance excitation of localized surface plasmons in nanoparticles of some metals. By adequate choice of shapes and sizes of metallic nanoparticles, kind of adsorbed molecules and wavelength of exciting light, it is possible to achieve enhancement of intensity of Raman scattering equal to 1012-1014. It means that only few molecules inside exciting light beam are sufficient to record the Raman spectrum. Then SERS can be very sensitive tools of trace analysis and very useful in recording Raman spectra of extremely low concentrated biomolecules. The MEF technique can become very useful in study of low fluorescent compounds. In contraire to SERS system for MEF has to be specially prepared to avoid quenching of fluorescence i.e. between the metal nanoparticles and fluorophores should exist the distance layer formed by dielectric or by adsorbed nonfluorescent macromolecules. Increase of fluorescence intensity of low fluorescent compound and possibility of record fluorescence of extremely low concentrated compound cause that MEF can become very promising techniques in biomedical research.

Paper Details

Date Published: 20 April 2006
PDF: 7 pages
Proc. SPIE 6158, Lightmetry and Light and Optics in Biomedicine 2004, 61580Q (20 April 2006); doi: 10.1117/12.675797
Show Author Affiliations
Stefan Kruszewski, Nicolaus Copernicus Univ. (Poland)
Henryk Malak, Univ. of Maryland Baltimore County (United States)
Blanka Ziomkowska, Nicolaus Copernicus Univ. (Poland)
Michał Cyrankiewicz, Nicolaus Copernicus Univ. (Poland)


Published in SPIE Proceedings Vol. 6158:
Lightmetry and Light and Optics in Biomedicine 2004

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