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Proceedings Paper

Use of optimization algorithmic techniques with active optics for aberration correction in optical sectioning microscopy
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Paper Abstract

Confocal techniques allow the user to achieve optically sectioned images with significantly enhanced axial and improved lateral resolution compared to widefield methods. Unfortunately, as one images more deeply within a sample, sample induced aberrations lead to a significant reduction in image resolution and contrast. Using adaptive optic techniques, we report on the effectiveness of a number of algorithms for removing sample induced aberrations. The viability and efficiency at a number of fitness parameters used in the optimisation routines is also considered.

Paper Details

Date Published: 8 June 2006
PDF: 9 pages
Proc. SPIE 6018, 5th International Workshop on Adaptive Optics for Industry and Medicine, 60181H (8 June 2006); doi: 10.1117/12.669388
Show Author Affiliations
S. P. Poland, Univ. of Strathclyde (United Kingdom)
D. Burns, Univ. of Strathclyde (United Kingdom)
W. Lubeigt, Univ. of Strathclyde (United Kingdom)
B. A. Patterson, Univ. of Strathclyde (United Kingdom)
G. Valentine, Univ. of Strathclyde (United Kingdom)
A. J. Wright, Univ. of Strathclyde (United Kingdom)
J. M. Girkin, Univ. of Strathclyde (United Kingdom)


Published in SPIE Proceedings Vol. 6018:
5th International Workshop on Adaptive Optics for Industry and Medicine

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