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Proceedings Paper

Optical enhanced luminescent measurements and sequential reagent mixing on a centrifugal microfluidic device for multi-analyte point-of-care applications
Author(s): Daniel A. Bartholomeusz; Rupert H. Davies; Joseph D. Andrade
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Paper Abstract

A centrifugal-based microfluidic device1 was built with lyophilized bioluminescent reagents for measuring multiple metabolites from a sample of less than 15 μL. Microfluidic channels, reaction wells, and valves were cut in adhesive vinyl film using a knife plotter with features down to 30 μm and transferred to metalized polycarbonate compact disks (CDs). The fabrication method was simple enough to test over 100 prototypes within a few months. It also allowed enzymes to be packaged in microchannels without exposure to heat or chemicals. The valves were rendered hydrophobic using liquid phase deposition. Microchannels were patterned using soft lithography to make them hydrophilic. Reagents and calibration standards were deposited and lyophilized in different wells before being covered with another adhesive film. Sample delivery was controlled by a modified CD ROM. The CD was capable of distributing 200 nL sample aliquots to 36 channels, each with a different set of reagents that mixed with the sample before initiating the luminescent reactions. Reflection of light from the metalized layer and lens configuration allowed for 20% of the available light to be collected from each channel. ATP was detected down to 0.1 μM. Creatinine, glucose, and galactose were also measured in micro and milliMolar ranges. Other optical-based analytical assays can easily be incorporated into the device design. The minimal sample size needed and expandability of the device make it easier to simultaneously measure a variety of clinically relevant analytes in point-of-care settings.

Paper Details

Date Published: 25 February 2006
PDF: 12 pages
Proc. SPIE 6080, Advanced Biomedical and Clinical Diagnostic Systems IV, 60800X (25 February 2006); doi: 10.1117/12.669226
Show Author Affiliations
Daniel A. Bartholomeusz, Univ. of Utah (United States)
Rupert H. Davies, Univ. of Washington (United States)
Joseph D. Andrade, Univ. of Utah (United States)

Published in SPIE Proceedings Vol. 6080:
Advanced Biomedical and Clinical Diagnostic Systems IV
Gerald E. Cohn; Warren S. Grundfest; David A. Benaron; Tuan Vo-Dinh, Editor(s)

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