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Proceedings Paper

Gene transfer of human hepatocyte growth factor by the use of nanosecond pulsed laser-induced stress waves
Author(s): Mitsuhiro Terakawa; Shunichi Sato; Daizoh Saitoh; Makoto Hasegawa; Hiroshi Ashida; Hideyuki Okano; Minoru Obara
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Paper Abstract

We successfully delivered a therapeutic vector construct, which carries hepatocyte growth factor (HGF) gene, to rat skin in vivo. After HGF expression vector had been intradermally injected to rat skin, LISWs were generated by irradiating the laser target put on the rat skin with nanosecond pulses from the second harmonics (532 nm) of a Q-switched Nd:YAG laser. Concentration of HGF protein increased by a factor of four by the application of LISWs when compared with that of control samples without LISW application. We also investigated the effects of LISWs on the integrity of plasmid DNA.

Paper Details

Date Published: 7 June 2006
PDF: 4 pages
Proc. SPIE 6261, High-Power Laser Ablation VI, 626133 (7 June 2006); doi: 10.1117/12.668965
Show Author Affiliations
Mitsuhiro Terakawa, Keio Univ. (Japan)
Shunichi Sato, National Defense Medical College Research Institute (Japan)
Daizoh Saitoh, National Defense Medical College Research Institute (Japan)
Makoto Hasegawa, Keio Univ. (Japan)
Hiroshi Ashida, National Defense Medical College Research Institute (Japan)
Hideyuki Okano, Keio Univ. (Japan)
Minoru Obara, Keio Univ. (Japan)


Published in SPIE Proceedings Vol. 6261:
High-Power Laser Ablation VI
Claude R. Phipps, Editor(s)

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