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Proceedings Paper

Local protein/gene density measurements using SMI microscopy
Author(s): Udo J. Birk; David Baddeley; Christoph Cremer
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Paper Abstract

Spatially Modulated Illumination (SMI) microscopy was applied to determine changes of the local refractive index at discrete fluorescently labeled sites within the cell nucleus. We present measurements on polymerase II complexes, where we found a variation of the local refractive index of 1.38 - 1.55 (standard deviation interval) throughout the nucleus. This variability is not correlated to the accumulations and the extensions of the polymerase II complexes, which have been determined in a previous experiment.1 Local protein accumulations such as adherent transcriptionally active proteins could possibly contribute to such variations, as could also different compactions of the DNA fiber. Altogether, we present a method to precisely obtain a map of the local refractive index inside of cell nuclei, which provides another contrasting mechanism for visualizing sub cellular structures.

Paper Details

Date Published: 27 April 2006
PDF: 5 pages
Proc. SPIE 6188, Optical Micro- and Nanometrology in Microsystems Technology, 61880W (27 April 2006); doi: 10.1117/12.668023
Show Author Affiliations
Udo J. Birk, Univ. Heidelberg (Germany)
David Baddeley, Univ. Heidelberg (Germany)
Christoph Cremer, Univ. Heidelberg (Germany)
The Jackson Lab. (United States)

Published in SPIE Proceedings Vol. 6188:
Optical Micro- and Nanometrology in Microsystems Technology
Christophe Gorecki; Anand K. Asundi; Wolfgang Osten, Editor(s)

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