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Proceedings Paper

Quantitative doping of commercial polystyrene microbeads with quantum dots
Author(s): Hai-Qiao Wang; Zhen-Li Huang; Tian-Cai Liu; Jian-Hao Wang; Yuan-Di Zhao; Qing-Ming Luo
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Paper Abstract

Microbeads with embedded encode characteristics are of considerable interest due to their potential use in multiplexed bioassays, high-throughput screening and combinatorial chemistry. Lots of encoding strategies for tagging or labeling microbeads have arisen in the past couple of years. Compared with the organic dye counterparts, the ideal optical properties of quantum dots (QDs) (e.g. characteristic narrow and symmetric spectra, size-tunable emission and simultaneous excitation) make it possible to tag microbeads in a quantitative way. In this paper, quantitative doping of commercial polystyrene microbeads with single color quantum dots was reported. A detailed analysis of the optical characteristics of the QD-tagged microbeads was presented based on the combination of fluorescent spectroscopy and microscope imaging.

Paper Details

Date Published: 2 February 2006
PDF: 10 pages
Proc. SPIE 6026, ICO20: Biomedical Optics, 60261J (2 February 2006); doi: 10.1117/12.667679
Show Author Affiliations
Hai-Qiao Wang, Huazhong Univ. of Science and Technology (China)
Zhen-Li Huang, Huazhong Univ. of Science and Technology (China)
Tian-Cai Liu, Huazhong Univ. of Science and Technology (China)
Jian-Hao Wang, Huazhong Univ. of Science and Technology (China)
Yuan-Di Zhao, Huazhong Univ. of Science and Technology (China)
Qing-Ming Luo, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 6026:
ICO20: Biomedical Optics
Gert von Bally; Qingming Luo, Editor(s)

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