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Proceedings Paper

Influence of different magnetic composites carriers on the immobilization of laccase
Author(s): Haiyan Xiao; Jun Huang; Bin Li; Juntao Wang; Desheng Jiang
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Paper Abstract

Laccase (E.C.1.10.3.2) has been used in various fields and enzyme immobilization technology is an effective means to perform enzyme reuse and to improve its stability. Carrier materials play an important role in the application of an immobilized enzyme. Magnetic carriers have been widely used in the field of protein and enzyme immobilization. The most important parameters of magnetic carriers are size, structure, density of reactive surface groups and the superparamagnetic property. The copper tetraaminophthalocyanine (CuTAPc)- Fe3O4 nano particle composite and chitosan-Fe3O4 microspheres composite were successfully synthesized and characterized by FTIR spectra, XRD and SEM micrograph. Active amino groups of two magnetic carriers could be used to bind laccase via glutaraldehyde. The optimal pH of the two immobilized laccases were the same at pH 3.0. The optimal temperature of laccase immobilized on CuTAPc-Fe3O4 nano particle was 45°C and that of the chitosan-Fe3O4 microspheres was 55°C. The immobilization yields of the two immobilized laccases were 5mg/g and 16mg/g, respectively. The Km value of the laccase immobilized on CuTAPc-Fe3O4 nano particles was 23.8μM, lower than that of the laccase immobilized on chitosan-Fe3O4 microspheres, 171.1μM. The laccase immobilized on magnetic composites could be used as biological sensing materials for biosensor.

Paper Details

Date Published: 2 February 2006
PDF: 8 pages
Proc. SPIE 6026, ICO20: Biomedical Optics, 60261G (2 February 2006); doi: 10.1117/12.667657
Show Author Affiliations
Haiyan Xiao, Wuhan Univ. of Technology (China)
Jun Huang, Wuhan Univ. of Technology (China)
Bin Li, Wuhan Univ. of Technology (China)
Juntao Wang, Wuhan Univ. of Technology (China)
Desheng Jiang, Wuhan Univ. of Technology (China)


Published in SPIE Proceedings Vol. 6026:
ICO20: Biomedical Optics
Gert von Bally; Qingming Luo, Editor(s)

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