Share Email Print
cover

Proceedings Paper

The influence of the complex refractive index of tissues on the image contrast of phase-contrast microscopy
Author(s): Rong Wang; Nai Zhou Xu; Ming Jie Wang
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Most of the materials in nature have complex refractive indexes, especially for biological tissues. The real and imaginary components of the complex refractive index are respectively responsible for the phase delay and absorption of light traveled in the tissues. Taking the complex refractive index into consideration, we modified the imaging theory of phase contrast microscopy. A relationship between the distributions of complex refractive index in measured samples and the intensity distributions of phase contrast micrographs is obtained. Based on this relationship, the influences of the refractive index and thickness of biological slices on the image contrast of phase contrast micrographs are analyzed in detail.

Paper Details

Date Published: 2 February 2006
PDF: 5 pages
Proc. SPIE 6026, ICO20: Biomedical Optics, 60260X (2 February 2006); doi: 10.1117/12.667416
Show Author Affiliations
Rong Wang, Nanjing Univ. of Science and Technology (China)
Nai Zhou Xu, Nanjing Univ. of Science and Technology (China)
Ming Jie Wang, Nanjing Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 6026:
ICO20: Biomedical Optics
Gert von Bally; Qingming Luo, Editor(s)

© SPIE. Terms of Use
Back to Top