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Proceedings Paper

Analysis of the effect on tissue autofluorescence detection from off-focus of linear-array pinholes in LP-laser scanning confocal fluorescence microscopical imaging spectrometer
Author(s): Xing Su; Weijian Tian; Chunmin Zhang
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Paper Abstract

The research of antofluorescence of the live tissue starts to be used in the disease diagnoses and prevention in recent years. The laser scanning confocal microscope has always played a great role in the autofluorescence research in biomedical field. As the technology of imaging spectrometer got successfully used in the remote sensing fields, microscopical imaging spectrometer for biomedical analyses emerged after the integration of imaging spectrometer and laser scanning confocal microscope. It can get the autofluorescence image and the spectral information of the sample simultaneously, this will supply more information to the diagnoses for difficult diseases. In this paper the principle of a Linear-array Pinholes Laser Scanning Confocal Microscopical Imaging Spectrometer (LP-LSCMIS) developed by the authors is proposed. It can be widely used in the autofluorescence detection of the live tissue in the body, such as the gastric, kidney, etc. The effect of off-focus of linear-array pinholes on microscopical imaging spectrometer subsystem in this device is mainly analyzed. The spatially resolution of fluorescence imaging and the modulation depth of autofluorescence spectrum in the live tissue is affected by the off-focus according to our research. We analyse the modulation depth of the interferogram thinking of the factor of off-focus.

Paper Details

Date Published: 2 February 2006
PDF: 7 pages
Proc. SPIE 6026, ICO20: Biomedical Optics, 60260S (2 February 2006); doi: 10.1117/12.667176
Show Author Affiliations
Xing Su, Xi'an Institute of Optics and Precision Mechanics (China)
Chinese Academy of Sciences (China)
Weijian Tian, Xi'an Institute of Optics and Precision Mechanics (China)
Chunmin Zhang, Xi'an Jiaotong Univ. (China)

Published in SPIE Proceedings Vol. 6026:
ICO20: Biomedical Optics
Gert von Bally; Qingming Luo, Editor(s)

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