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Proceedings Paper

Detection of biological particles in ambient air using bio-aerosol mass spectrometry
Author(s): Erica L. McJimpsey; Paul T. Steele; Keith R. Coffee; David P. Fergenson; Vincent J. Riot; Bruce W. Woods; Eric E. Gard; Matthias Frank; Herbert J. Tobias; Carlito Lebrilla
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Paper Abstract

The Bio-Aerosol Mass Spectrometry (BAMS) system is an instrument used for the real time detection and identification of biological aerosols. Particles are drawn from the atmosphere directly into vacuum and tracked as they scatter light from several continuous wave lasers. After tracking, the fluorescence of individual particles is excited by a pulsed 266nm or 355nm laser. Molecules from those particles with appropriate fluorescence properties are subsequently desorbed and ionized using a pulsed 266nm laser. Resulting ions are analyzed in a dual polarity mass spectrometer. During two field deployments at the San Francisco International Airport, millions of ambient particles were analyzed and a small but significant fraction were found to have fluorescent properties similar to Bacillus spores and vegetative cells. Further separation of non-biological background particles from potential biological particles was accomplished using laser desorption/ionization mass spectrometry. This has been shown to enable some level of species differentiation in specific cases, but the creation and observation of higher mass ions is needed to enable a higher level of specificity across more species. A soft ionization technique, matrix-assisted laser desorption/ionization (MALDI) is being investigated for this purpose. MALDI is particularly well suited for mass analysis of biomolecules since it allows for the generation of molecular ions from large mass compounds that would fragment under normal irradiation. Some of the initial results from a modified BAMS system utilizing this technique are described.

Paper Details

Date Published: 19 May 2006
PDF: 10 pages
Proc. SPIE 6218, Chemical and Biological Sensing VII, 62180B (19 May 2006); doi: 10.1117/12.666459
Show Author Affiliations
Erica L. McJimpsey, Lawrence Livermore National Lab. (United States)
Univ. of California, Davis (United States)
Paul T. Steele, Lawrence Livermore National Lab. (United States)
Keith R. Coffee, Lawrence Livermore National Lab. (United States)
David P. Fergenson, Lawrence Livermore National Lab. (United States)
Vincent J. Riot, Lawrence Livermore National Lab. (United States)
Bruce W. Woods, Lawrence Livermore National Lab. (United States)
Eric E. Gard, Lawrence Livermore National Lab. (United States)
Matthias Frank, Lawrence Livermore National Lab. (United States)
Herbert J. Tobias, Lawrence Livermore National Lab. (United States)
Carlito Lebrilla, Univ. of California, Davis (United States)

Published in SPIE Proceedings Vol. 6218:
Chemical and Biological Sensing VII
Patrick J. Gardner; Augustus W. Fountain, Editor(s)

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