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Proceedings Paper

Phage-based magnetostrictive-acoustic microbiosensors for detecting Bacillus anthracis spores
Author(s): J. Wan; H. Yang; R. S. Lakshmanan; R. Guntupalli; S. Huang; J. Hu; V. A. Petrenko; B. A. Chin
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Paper Abstract

Magnetostrictive particles (MSPs) as biosensor platform have been developed recently. The principle of MSPs as sensor platform is the same as that of other acoustic wave devices, such as quartz crystal microbalance. In this paper, the fabrication, characterization and performance of phage-based MSP biosensors for detecting Bacillus anthracis spores are reported. A commercially available magnetostrictive alloy was utilized to fabricate the sensor platform. The phage was immobilized onto the MSPs using physical adsorption technology. The following performance of the phage-based MSP sensors will be presented: sensitivity, response time, longevity, specificity and binding efficacy. The performance of the sensors at static and dynamic conditions was characterized. The experimental results are confirmed by microscopy photographs. The excellent performance including high sensitivity and rapid response is demonstrated. More importantly, it is experimentally found that the phage-based MSP sensors have a much better longevity than antibody-based sensors.

Paper Details

Date Published: 18 May 2006
PDF: 9 pages
Proc. SPIE 6223, Micro (MEMS) and Nanotechnologies for Space Applications, 62230E (18 May 2006); doi: 10.1117/12.666205
Show Author Affiliations
J. Wan, Auburn Univ. (United States)
H. Yang, Auburn Univ. (United States)
R. S. Lakshmanan, Auburn Univ. (United States)
R. Guntupalli, Auburn Univ. (United States)
S. Huang, Auburn Univ. (United States)
J. Hu, Auburn Univ. (United States)
V. A. Petrenko, Auburn Univ. (United States)
B. A. Chin, Auburn Univ. (United States)

Published in SPIE Proceedings Vol. 6223:
Micro (MEMS) and Nanotechnologies for Space Applications
Thomas George; Zhong-Yang Cheng, Editor(s)

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