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Proceedings Paper

Detection of Salmonella typhimurium using polyclonal antibody immobilized magnetostrictive biosensors
Author(s): R. Guntupalli; Jing Hu; Ramji S. Lakshmanan; Jiehui Wan; Shichu Huang; Hong Yang; James M. Barbaree; T. S. Huang; Bryan A. Chin
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Paper Abstract

Novel mass-sensitive, magnetostrictive sensors have a characteristic resonant frequency that can be determined by monitoring the magnetic flux emitted by the sensor in response to an applied, time varying, magnetic field. This magnetostrictive platform has a unique advantage over conventional sensor platforms in that measurement is wireless or remote. These biosensors can thus be used in-situ for detecting pathogens and biological threat agents. In this work, we have used a magnetostrictive platform immobilized with a polyclonal antibody (the bio-molecular recognition element) to form a biosensor for the detection of Salmonella typhimurium. Upon exposure to solutions containing Salmonella typhimurium bacteria, the bacteria were bound to the sensor and the additional mass of the bound bacteria caused a shift in the sensor's resonant frequency. Responses of the sensors to different concentrations of S. typhimurium were recorded and the results correlated with those obtained from scanning electron microscopy (SEM) images of samples. Good agreement between the measured number of bound bacterial cells (attached mass) and frequency shifts were obtained. The longevity and specificity of the selected polyclonal antibody were also investigated and are reported.

Paper Details

Date Published: 10 May 2006
PDF: 9 pages
Proc. SPIE 6201, Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense V, 62010P (10 May 2006); doi: 10.1117/12.665919
Show Author Affiliations
R. Guntupalli, Auburn Univ. (United States)
Jing Hu, Auburn Univ. (United States)
Ramji S. Lakshmanan, Auburn Univ. (United States)
Jiehui Wan, Auburn Univ. (United States)
Shichu Huang, Auburn Univ. (United States)
Hong Yang, Auburn Univ. (United States)
James M. Barbaree, Auburn Univ. (United States)
T. S. Huang, Auburn Univ. (United States)
Bryan A. Chin, Auburn Univ. (United States)

Published in SPIE Proceedings Vol. 6201:
Sensors, and Command, Control, Communications, and Intelligence (C3I) Technologies for Homeland Security and Homeland Defense V
Edward M. Carapezza, Editor(s)

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