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Proceedings Paper

Single quantum dot imaging in live cells: toward a cellular GPS
Author(s): Sébastien Courty; Marcel Zevenbergen; Cédric Bouzigues; Marie-Virginie Ehrensperger; Camilla Luccardini; Assa Sittner; Stéphane Bonneau; Maxime Dahan
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Paper Abstract

Colloidal semiconductor quantum dots (QDs) have become common fluorescent probes in biology. Their optical properties not only facilitate spectrally multiplexed detection but also enable single molecule measurements with high signal to noise ratio. This is of particular interest in cell biology since it allows individual QD-tagged biomolecules to be tracked with good spatial and temporal resolution over long durations. Recent measurements on membrane proteins have validated this approach and serve as a basis for more complex experiments in which the motion of different biomolecules, located in various cell compartments (membrane, cytosol, nucleus,...) and tagged with QDs having distinct emission colors, is recorded in real time and with a nanometer resolution. The development of these new imaging methods, equivalent to a molecular positioning system within a single cell, raises many challenges, coming from optics, physical and biological chemistry, as well as image processing.

Paper Details

Date Published: 27 March 2006
PDF: 7 pages
Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60960W (27 March 2006); doi: 10.1117/12.663348
Show Author Affiliations
Sébastien Courty, École Normale Supérieure (France)
Univ. Pierre et Marie Curie (France)
Marcel Zevenbergen, École Normale Supérieure (France)
Univ. Pierre et Marie Curie (France)
Cédric Bouzigues, École Normale Supérieure (France)
Univ. Pierre et Marie Curie (France)
Marie-Virginie Ehrensperger, École Normale Supérieure (France)
Univ. Pierre et Marie Curie (France)
Camilla Luccardini, École Normale Supérieure (France)
Univ. Pierre et Marie Curie (France)
Assa Sittner, École Normale Supérieure (France)
Univ. Pierre et Marie Curie (France)
Stéphane Bonneau, École Normale Supérieure (France)
Univ. Pierre et Marie Curie (France)
Maxime Dahan, École Normale Supérieure (France)
Univ. Pierre et Marie Curie (France)


Published in SPIE Proceedings Vol. 6096:
Colloidal Quantum Dots for Biomedical Applications
Marek Osinski; Kenji Yamamoto; Thomas M. Jovin, Editor(s)

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