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Proceedings Paper

Probing the effects of spectral overlap on quantum-dot-based FRET: Ensemble and single molecule studies
Author(s): Thomas Pons; Igor L. Medintz; Hedi Mattoussi
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Paper Abstract

The photoluminescence spectrum of a quasi-monodisperse semiconductor quantum dot (QD) population is composed of a continuum of extremely narrow single QD spectra. This is due to inhomogeneities in nanocrystal size within a population and the color-size dependency imposed by effects of carrier quantum confinements. We take advantage of this population heterogeneity to gain a unique insight into the fluorescence resonant energy transfer (FRET) process between a QD donor and proximal dye-labeled protein acceptors. Our ensemble and single-QD studies demonstrate that the spectral dependency of the energy transfer rate matches the acceptor absorption spectrum as predicted by Forster formalism. This allows ratiometric FRET measurements based on the QD donor emission.

Paper Details

Date Published: 27 March 2006
PDF: 9 pages
Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60960L (27 March 2006); doi: 10.1117/12.663321
Show Author Affiliations
Thomas Pons, Naval Research Lab. (United States)
Johns Hopkins Univ. (United States)
Igor L. Medintz, Naval Research Lab. (United States)
Hedi Mattoussi, Naval Research Lab. (United States)

Published in SPIE Proceedings Vol. 6096:
Colloidal Quantum Dots for Biomedical Applications
Marek Osinski; Kenji Yamamoto; Thomas M. Jovin, Editor(s)

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