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Proceedings Paper

Optimising a grating-coupled evanescent field excitation
Author(s): K. Kolari; A. Hokkanen; M. Kuittinen; J. Simonen; P. Heimala
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Paper Abstract

Diffraction gratings were designed and fabricated on a SiN/SiO2 planar waveguide to couple light from a low power 488 nm laser beam into the planar waveguide. The light propagating in the waveguide was then used to detect fluoresceine from volume on the planar waveguide surface. The results demonstrate the capability for very simple and fast analytical throughput for quantification of fluorescent samples, essentially without cross-talk. The transmission measurements show about 10% diffraction efficiency with 0.06° FWHM. The diffraction efficiency and the incidence angle for the maximum diffraction efficiency were observed to be highly dependent on the process parameters used to fabricate the gratings. The fluorescence signal was observed to be linear for fluoresceine concentrations between 10-9 and 10-3 M.

Paper Details

Date Published: 22 April 2006
PDF: 12 pages
Proc. SPIE 6189, Optical Sensing II, 61891Z (22 April 2006); doi: 10.1117/12.662622
Show Author Affiliations
K. Kolari, VTT Information Technology (Finland)
A. Hokkanen, VTT Information Technology (Finland)
M. Kuittinen, Univ. of Joensuu (Finland)
J. Simonen, Univ. of Joensuu (Finland)
P. Heimala, VTT Information Technology (Finland)


Published in SPIE Proceedings Vol. 6189:
Optical Sensing II
Brian Culshaw; Anna G. Mignani; Hartmut Bartelt; Leszek R. Jaroszewicz, Editor(s)

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