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Proceedings Paper

Monitoring apoptosis of TK-GFP-expressing ACC-M cells induced by ACV using FRET technique
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Paper Abstract

Apoptosis is an evolutionary conserved cellular process that plays an important role during development, but it is also involved in tissue homeostasis and in many diseases. To study the characteristics of suicide gene system of the herpes simplex virus thymidine kinase (HSV-tk) gene in tumor cells and explore the apoptosis phenomena in this system and its effect on the human adenoid cystic carcinoma line ACC-M cell, we detected apoptosis of CD3- (ECFP-CRS-DsRed) and TK-GFP-expressing ACC-M (ACC-M-TK-GFP-CD3) cells induced by acyclovir (ACV) using fluorescence resonance energy transfer (FRET) technique. CD3 is a FRET-based indicator for activity of caspase-3, which is composed of an enhanced cyan fluorescent protein, a caspase-3 sensitive linker, and a red fluorescent protein from Discosoma with efficient maturation property. FRET from ECFP to DsRed could be detected in normal ACC-M-TK-GFP-CD3 cells, and the FRET efficient was remarkably decreased and then disappeared during the cells apoptosis induced by ACV. It was due to the activated caspase-3 cleaved the CD3 fusion protein. In this study, the results suggested that the ACV-induced apoptosis of ACC-M-TK-GFP-CD3 cells was through caspase-3 pathway.

Paper Details

Date Published: 19 May 2006
PDF: 5 pages
Proc. SPIE 6218, Chemical and Biological Sensing VII, 62180L (19 May 2006); doi: 10.1117/12.662591
Show Author Affiliations
Tao Xiong, Huazhong Univ. of Science and Technology (China)
China Univ. of Geosciences (China)
Zhihong Zhang, Huazhong Univ. of Science and Technology (China)
Juqiang Lin, Huazhong Univ. of Science and Technology (China)
Jie Yang, Huazhong Univ. of Science and Technology (China)
Shaoqun Zeng, Huazhong Univ. of Science and Technology (China)
Qingming Luo, Huazhong Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 6218:
Chemical and Biological Sensing VII
Patrick J. Gardner; Augustus W. Fountain, Editor(s)

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