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Proceedings Paper

Active locking of adaptive optics for improved microscopy
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Paper Abstract

We present a method for aberration correction in a confocal microscope that successfully combines both a spatial light modulator and a deformable membrane mirror. An active locking technique is used that benefits from the fast update rate of the deformable membrane mirror and the large effective stroke of the spatial light modulator. Concentrating on defocus, we were able to track 'best focus' over a distance of 80 μm with a lock RMS precision of 57 nm. In principle, this technique can be applied to any Zernike mode or aberration that can be accurately reproduced on the deformable membrane mirror.

Paper Details

Date Published: 14 April 2006
PDF: 8 pages
Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61910F (14 April 2006); doi: 10.1117/12.662555
Show Author Affiliations
Amanda J. Wright, Univ. of Strathclyde (United Kingdom)
Graham M. Gibson, Univ. of Glasgow (United Kingdom)
Simon P. Poland, Univ. of Strathclyde (United Kingdom)
Brett A. Patterson, Univ. of Strathclyde (United Kingdom)
Miles J. Padgett, Univ. of Glasgow (United Kingdom)
John M. Girkin, Univ. of Strathclyde (United Kingdom)


Published in SPIE Proceedings Vol. 6191:
Biophotonics and New Therapy Frontiers
Romualda Grzymala; Olivier Haeberle, Editor(s)

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