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Proceedings Paper

Wavefront engineering microscopy to study 3D mechanotransduction in living cells
Author(s): Valentina Emiliani; Dan Cojoc; Enrico Ferrari; Valeria Garbin; Christiane Durieux; Enzo Di Fabrizio
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Paper Abstract

We present an experimental system based on the use of a spatial light modulator which enables to perform simultaneously 3D optical manipulation and optical sectioning. This has been achieved by modifying the wave front of the trapping beam with properly diffractive optical elements displayed on a computer controlled spatial light modulator. We demonstrated the capability of the system in two experimental schemes, in a first one we performed a 3D optical scanning of 6 trapped beads by displacing the beads through a fixed imaging plane. In a second one we scan the imaging plane and simultaneously compensate for the movement of the objective in order to keep the trapping plane at a fixed position.

Paper Details

Date Published: 20 April 2006
PDF: 8 pages
Proc. SPIE 6195, Nanophotonics, 61950J (20 April 2006); doi: 10.1117/12.662531
Show Author Affiliations
Valentina Emiliani, Institut Jacques Monod, CNRS, Univ. P6/P7 (France)
Dan Cojoc, INFM-TASC, IILIT Beamline at Elettra Sincrotrone (Italy)
Enrico Ferrari, INFM-TASC, IILIT Beamline at Elettra Sincrotrone (Italy)
Valeria Garbin, INFM-TASC, IILIT Beamline at Elettra Sincrotrone (Italy)
Christiane Durieux, Institut Jacques Monod, CNRS, Univ. P6/P7 (France)
Enzo Di Fabrizio, INFM-TASC, IILIT Beamline at Elettra Sincrotrone (Italy)


Published in SPIE Proceedings Vol. 6195:
Nanophotonics
David L. Andrews; Jean-Michel Nunzi; Andreas Ostendorf, Editor(s)

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