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Proceedings Paper

Characterization of single functionalized quantum dots using combined atomic force and confocal fluorescence microscopy
Author(s): Dal-Hyun Kim; Kenji Okamoto; Lori S. Goldner; Jeeseong Hwang
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Paper Abstract

Fluorescent nanocrystals (quantum dots or QDs) have a number of unique properties that overcome the limitations of conventional organic dyes. However, the optical properties of QDs have been observed to be strongly dependent upon their local chemical environment including novel surface coatings, which have been developed to render QDs water soluble and conjugation ready leading to their use as fluorescent tags and optical sensors for a variety of biological and biomedical applications. The quantitative utility of QDs in complex biological systems requires that their optical properties be well understood and interrelated with their chemical functionalization on the surface and their interactions with surface-conjugated materials. In this report, quantitative measurement of adhesion forces between a hydrophilic or a hydrophobic AFM probe and an amine-functionalized single QD or a hydrophilic substrate were obtained to demonstrate the utility of the atomic force microscopy (AFM) as a tool to probe surface functionalities of single functionalized QDs. We also present procedures to combine AFM and confocal fluorescence microscopy in an effort to simultaneously probe optical characteristics and physical/chemical properties of single or clustered functionalized QDs at the nanoscale.

Paper Details

Date Published: 27 March 2006
PDF: 8 pages
Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60960G (27 March 2006); doi: 10.1117/12.661784
Show Author Affiliations
Dal-Hyun Kim, National Institute of Standards and Technology (United States)
Kenji Okamoto, National Institute of Standards and Technology (United States)
Lori S. Goldner, National Institute of Standards and Technology (United States)
Jeeseong Hwang, National Institute of Standards and Technology (United States)


Published in SPIE Proceedings Vol. 6096:
Colloidal Quantum Dots for Biomedical Applications
Marek Osinski; Kenji Yamamoto; Thomas M. Jovin M.D., Editor(s)

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