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Proceedings Paper

Hybrid TiO2 nanoparticles: an approach for developing site specific DNA cleavage
Author(s): J. Liu; Z. Saponjic; N. M. Dimitrijevic; S. Luo; D. Preuss; T. Rajh
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Paper Abstract

We have developed hybrid light responsive TiO2 nanoparticles electronically linked to PNA oligonucleotides that site specifically bind to double stranded target DNA. This opens a new opportunity for the development of a highly efficient "artificial restriction enzyme" whose activity can be controlled by using light. The work focuses on the use of TiO2 nanocomposites as analogs of restriction enzymes with unique specificity that does not exist in current biological approaches. TiO2 nanoparticles electronically linked to DNA or PNA adapters have been site-specifically attached along double stranded λ DNA vectors. Illumination of this assembly results in selective oxidation of DNA at the deepest "thermodynamic traps" located closest to the nanoparticle surface, causing DNA cleavage. We investigate the effect of the sequence and length of DNA and PNA adapters on the specificity of DNA cleavage. Related to this issue, the potential use of TiO2/DNA nanocomposites as "rare cutters" that cleave DNA in the places not achieved with existing protein-based enzymes is investigated.

Paper Details

Date Published: 27 March 2006
PDF: 10 pages
Proc. SPIE 6096, Colloidal Quantum Dots for Biomedical Applications, 60960F (27 March 2006); doi: 10.1117/12.661778
Show Author Affiliations
J. Liu, Argonne National Lab. (United States)
Z. Saponjic, Argonne National Lab. (United States)
N. M. Dimitrijevic, Argonne National Lab. (United States)
S. Luo, Univ. of Chicago (United States)
D. Preuss, Howard Hughes Medical Institute, Univ. of Chicago (United States)
T. Rajh, Argonne National Lab. (United States)

Published in SPIE Proceedings Vol. 6096:
Colloidal Quantum Dots for Biomedical Applications
Marek Osinski; Kenji Yamamoto; Thomas M. Jovin, Editor(s)

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