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Proceedings Paper

A novel optical detection system for chromatography applications
Author(s): W. Meulebroeck; H. Ottevaere; K. Scheir; D. Clicq; G. Desmet; H. Thienpont
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Paper Abstract

In this work we describe the design of a confocal microscope based optical system which can be used for two types of miniaturized fluid-chromatographic experiments: first of all for the study of shear-driven fluid separations in microchannels and secondly for the study of peak-broadening phenomena in pressure-driven fluids in the same type of microchannels. For the first type of measurements fluorescing dyes (coumarins) are used while for the latter type photo-active molecules (uncaged dyes) like for example fluorescein molecules are needed. We designed the detection system in such a way that by making some small changes to the system we can swap from one application to the other. After calculating the specifications of the optical components, we simulated the system with the sequential ray-tracing-software Solstis and built the system. Finally we did some preliminary experiments which demonstrated the working principle of our setup.

Paper Details

Date Published: 27 April 2006
PDF: 12 pages
Proc. SPIE 6188, Optical Micro- and Nanometrology in Microsystems Technology, 61881I (27 April 2006); doi: 10.1117/12.660763
Show Author Affiliations
W. Meulebroeck, Vrije Univ. Brussel (Belgium)
H. Ottevaere, Vrije Univ. Brussel (Belgium)
K. Scheir, Vrije Univ. Brussel (Belgium)
D. Clicq, Vrije Univ. Brussel (Belgium)
G. Desmet, Vrije Univ. Brussel (Belgium)
H. Thienpont, Vrije Univ. Brussel (Belgium)

Published in SPIE Proceedings Vol. 6188:
Optical Micro- and Nanometrology in Microsystems Technology
Christophe Gorecki; Anand K. Asundi; Wolfgang Osten, Editor(s)

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