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Proceedings Paper

Elastic scattering spectroscopy findings in formalin–fixed oral squamous cell carcinoma specimens
Author(s): B. Swinson; M. Elmaaytah; W. Jerjes; C. Hopper
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Paper Abstract

Oral squamous cell carcinoma (OSCC) has been shown to spread locally and infiltrate adjacent bone or via the lymphatic system to the cervical lymph nodes. This usually necessitates a surgical neck dissection and either a local or segmental resection for bone clearance. While histopathology remains the gold standard for tissue diagnosis, several new diagnostic techniques are being developed that rely on physical and biochemical changes that mirror or precede malignant changes within tissue. The aim of this study was to compare findings of Elastic Scattering Spectroscopy (ESS) with histopathology on formalin-fixed specimens of both neck lymph node dissections and de-calcified archival bone from patients with OSCC. We wished to see if this technique could be used as an adjunct or alternative to histopathology in defining cervical nodal involvement and if it could be used to identify bone resection margins positive for tumour. 130 lymph nodes were examined from 13 patients. The nodes were formalin-fixed, bivalved and examined by ESS. The intensity of the spectrum at 4 points was considered for comparison; at 360nm, 450nm, 630nm and 690nm. 341 spectra were taken from the mandibular specimens of 21 patients, of which 231 spectra were taken from histologically positive sites and the rest were normal. The nodes and bone specimens were then routinely processed with haematoxylin and eosin-stained sections, examined histopathologically, and the results compared. Using Linear Discriminant Analysis (LDA) as a statistical method, a sensitivity of 98% and a specificity of 68% was obtained for the neck nodes and a sensitivity of 87% and a specificity of 80% for the bone margins.

Paper Details

Date Published: 25 October 2005
PDF: 8 pages
Proc. SPIE 5968, Laser Florence 2004: A Window on the Laser Medicine World, 59680M (25 October 2005); doi: 10.1117/12.660077
Show Author Affiliations
B. Swinson, Eastman Dental Institute for Oral Healthcare Sciences (United Kingdom)
M. Elmaaytah, Eastman Dental Institute for Oral Healthcare Sciences (United Kingdom)
W. Jerjes, Eastman Dental Institute for Oral Healthcare Sciences (United Kingdom)
C. Hopper, Eastman Dental Institute for Oral Healthcare Sciences (United Kingdom)
Univ. College London Hospitals (United Kingdom)


Published in SPIE Proceedings Vol. 5968:
Laser Florence 2004: A Window on the Laser Medicine World
Leonardo Longo; Khalil A. Khatri; Mihail-Lucian Pascu; Wilhelm R.A. Waidelich, Editor(s)

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