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Proceedings Paper

Ultrafast third harmonic micro-spectroscopy reveals a two-photon resonance in human hemoglobin
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Paper Abstract

The recently developed technique of ultrafast third harmonic generation (THG) micro-spectroscopy is discussed. The approach is easily adapted to a standard laser scanning microscope and allows for two and three photon resonances to be identified in non-fluorescent unlabeled samples. This work provides nonlinear microscopists with a tool for further understanding the contrast and damage mechanisms they will encounter under nonlinear excitation. Here, we use THG micro-spectroscopy to investigate the nonlinear optical properties of hemoglobin over the spectral range of 770 -1000 nm with 100-fs duration, ~1-nJ energy laser pulses. We demonstrate the ability of this approach to distinguish different ligand binding states in physiological solutions of human hemoglobin.

Paper Details

Date Published: 28 February 2006
PDF: 8 pages
Proc. SPIE 6108, Commercial and Biomedical Applications of Ultrafast Lasers VI, 610809 (28 February 2006); doi: 10.1117/12.659110
Show Author Affiliations
Omar Clay, Univ. of California, San Diego (United States)
Chris B. Schaffer, Univ. of California, San Diego (United States)
Jeffrey A. Squier, Colorado School of Mines (United States)
David Kleinfeld, Univ. of California, San Diego (United States)

Published in SPIE Proceedings Vol. 6108:
Commercial and Biomedical Applications of Ultrafast Lasers VI
Joseph Neev; Stefan Nolte; Alexander Heisterkamp; Christopher B. Schaffer, Editor(s)

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