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Proceedings Paper

Laser-activated bubbles in living cells: new universal cytometric method
Author(s): Dmitri Lapotko; Ekaterina Lukianova
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Paper Abstract

Laser-activated micro- and nano-bubbles (LAB) in cells may be used as universal and sensitive probes for measuring properties of individual cells. Such bubbles can be detected and imaged by using microscopy and flow cytometry. LABs in living blood and tumor cells were induced by pulsed (532 nm, 10 ns) laser radiation and were detected by a thermal lens optical method. LAB lifetime and maximal diameter varied, correspondingly, within the ranges 0.02-10 ms and 0.44-100 mm. LAB parameters - thresholds and probabilities - were found to depend upon the physiological state of cells. Specificity and sensitivity of LAB cytometry were increased by using light-absorbing nanoparticles conjugated to specific monoclonal antibodies.

Paper Details

Date Published: 21 February 2006
PDF: 9 pages
Proc. SPIE 6088, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV, 608818 (21 February 2006); doi: 10.1117/12.658160
Show Author Affiliations
Dmitri Lapotko, Luikov Heat and Mass Transfer Institute (Belarus)
Ekaterina Lukianova, Luikov Heat and Mass Transfer Institute (Belarus)


Published in SPIE Proceedings Vol. 6088:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues IV
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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