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Proceedings Paper

Control the number of biomolecules tethering on functionalized AFM tips
Author(s): Z. D. Xiao; X. X. Xiao; Z. H. Lu; C. M. Yam; C. Z. Cai
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Paper Abstract

We have developed a practical method to control the number of attaching biomolecules to an AFM tip. Monolayer of OEG that has little interaction with some biomolecules was self-assembled on tips. Electric pulses were used to oxidize a patch of OEG monolayer on tip and generate COOH groups for further linking at the activated area. The surface groups were detected by chemical force titration. The total numbers of molecule bound on the tip was examined by the studies of biotin/streptavidin system.

Paper Details

Date Published: 27 February 2006
PDF: 5 pages
Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920Q (27 February 2006); doi: 10.1117/12.657867
Show Author Affiliations
Z. D. Xiao, Southeast Univ. (China)
X. X. Xiao, Southeast Univ. (China)
Z. H. Lu, Southeast Univ. (China)
C. M. Yam, Univ. of Houston (United States)
C. Z. Cai, Univ. of Houston (United States)


Published in SPIE Proceedings Vol. 6092:
Ultrasensitive and Single-Molecule Detection Technologies
Jörg Enderlein; Zygmunt K. Gryczynski, Editor(s)

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