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Proceedings Paper

Photoactivation in green to red converting EosFP and other fluorescent proteins from the GFP family
Author(s): Jörg Wiedenmann; G. Ulrich Nienhaus
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Paper Abstract

The green fluorescent protein (GFP) from the hydromedusa Aequorea victoria and its derivatives have become indispensable imaging devices in cell biology. In previous years, a wide variety of GFP-like proteins were discovered in non-bioluminescent anthozoa. Some of them displayed exciting new properties, including photoactivated changes of the fluorescence emission intensity and wavelength. Photoactivatable proteins offer a high potential as tools for regional optical marking in live cells and tissues. This review aims to give an overview of photoactivatable marker proteins, focusing on the molecular basis of light-induced green to red photoconversion in EosFP.

Paper Details

Date Published: 22 February 2006
PDF: 9 pages
Proc. SPIE 6098, Genetically Engineered Probes for Biomedical Applications, 609801 (22 February 2006); doi: 10.1117/12.657565
Show Author Affiliations
Jörg Wiedenmann, Univ. of Ulm (Germany)
G. Ulrich Nienhaus, Univ. of Ulm (Germany)
Univ. of Illinois at Urbana-Champaign (United States)

Published in SPIE Proceedings Vol. 6098:
Genetically Engineered Probes for Biomedical Applications
Alexander P. Savitsky; Rebekka M. Wachter, Editor(s)

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