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Proceedings Paper

Femtosecond laser dissection in C. elegans neural circuits
Author(s): Aravinthan D. T. Samuel; Samuel H. Chung; Damon A. Clark; Christopher V. Gabel; Chieh Chang; Venkatesh Murthy; Eric Mazur
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Paper Abstract

The nematode C. elegans, a millimeter-long roundworm, is a well-established model organism for studies of neural development and behavior, however physiological methods to manipulate and monitor the activity of its neural network have lagged behind the development of powerful methods in genetics and molecular biology. The small size and transparency of C. elegans make the worm an ideal test-bed for the development of physiological methods derived from optics and microscopy. We present the development and application of a new physiological tool: femtosecond laser dissection, which allows us to selectively ablate segments of individual neural fibers within live C. elegans. Femtosecond laser dissection provides a scalpel with submicrometer resolution, and we discuss its application in studies of neural growth, regenerative growth, and the neural basis of behavior.

Paper Details

Date Published: 10 February 2006
PDF: 6 pages
Proc. SPIE 6108, Commercial and Biomedical Applications of Ultrafast Lasers VI, 610801 (10 February 2006); doi: 10.1117/12.657396
Show Author Affiliations
Aravinthan D. T. Samuel, Harvard Univ. (United States)
Samuel H. Chung, Harvard Univ. (United States)
Damon A. Clark, Harvard Univ. (United States)
Christopher V. Gabel, Harvard Univ. (United States)
Chieh Chang, McGill Univ. (Canada)
Venkatesh Murthy, Harvard Univ. (United States)
Eric Mazur, Harvard Univ. (United States)

Published in SPIE Proceedings Vol. 6108:
Commercial and Biomedical Applications of Ultrafast Lasers VI
Joseph Neev; Stefan Nolte; Alexander Heisterkamp; Christopher B. Schaffer, Editor(s)

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