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Proceedings Paper

Registration and 3D visualization of large microscopy images
Author(s): Kishore Mosaliganti; Tony Pan; Richard Sharp; Randall Ridgway; Srivathsan Iyengar; Alexandra Gulacy; Pamela Wenzel; Alain de Bruin; Raghu Machiraju; Kun Huang; Gustavo Leone; Joel Saltz
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Paper Abstract

Inactivation of the retinoblastoma gene in mouse embryos causes tissue infiltrations into critical sections of the placenta, which has been shown to affect fetal survivability. Our collaborators in cancer genetics are extremely interested in examining the three dimensional nature of these infiltrations given a stack of two dimensional light microscopy images. Three sets of wildtype and mutant placentas was sectioned serially and digitized using a commercial light microscopy scanner. Each individual placenta dataset consisted of approximately 1000 images totaling 700 GB in size, which were registered into a volumetric dataset using National Library of Medicine's (NIH/NLM) Insight Segmentation and Registration Toolkit (ITK). This paper describes our method for image registration to aid in volume visualization of tissue level intermixing for both wildtype and Rb- specimens. The registration process faces many challenges arising from the large image sizes, damages during sectioning, staining gradients both within and across sections, and background noise. These issues limit the direct application of standard registration techniques due to frequent convergence to local solutions. In this work, we develop a mixture of automated and semi-automated enhancements with ground-truth validation for the mutual information-based registration algorithm. Our final volume renderings clearly show tissue intermixing differences between both wildtype and Rb- specimens which are not obvious prior to registration.

Paper Details

Date Published: 10 March 2006
PDF: 12 pages
Proc. SPIE 6144, Medical Imaging 2006: Image Processing, 61442V (10 March 2006); doi: 10.1117/12.653505
Show Author Affiliations
Kishore Mosaliganti, The Ohio State Univ. (United States)
Tony Pan, The Ohio State Univ. (United States)
Richard Sharp, The Ohio State Univ. (United States)
Randall Ridgway, The Ohio State Univ. (United States)
Srivathsan Iyengar, The Ohio State Univ. (United States)
Alexandra Gulacy, The Ohio State Univ. (United States)
Pamela Wenzel, The Ohio State Univ. (United States)
Alain de Bruin, The Ohio State Univ. (United States)
Raghu Machiraju, The Ohio State Univ. (United States)
Kun Huang, The Ohio State Univ. (United States)
Gustavo Leone, The Ohio State Univ. (United States)
Joel Saltz, The Ohio State Univ. (United States)


Published in SPIE Proceedings Vol. 6144:
Medical Imaging 2006: Image Processing
Joseph M. Reinhardt; Josien P. W. Pluim, Editor(s)

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