Share Email Print
cover

Proceedings Paper

Multipopulation desaggregation behavior of zFP538 upon dilution
Author(s): Vadim A. Korolenko; Evgeny Evtushenko; Alexander L. Rusanov; Nadya Zubova; Ilya N. Kurochkin; Alexander P. Savitsky
Format Member Price Non-Member Price
PDF $14.40 $18.00
cover GOOD NEWS! Your organization subscribes to the SPIE Digital Library. You may be able to download this paper for free. Check Access

Paper Abstract

Practically all fluorescent proteins from corals are oligomerized or aggregated in solutions. For zFP538 it was demonstrated that intensity of fluorescence and pH-profile change upon dilution that was explained by the dissociation of aggregates. Particle size distribution of zFP538 aggregates adsorbed on the surface of graphite from solutions in the concentration range 10-7 - 10-9 M was studied by AFM. Multi-population size distribution was observed for all three protein concentrations. Within one concentration, size distribution is characterized by three populations: small, medium, and large. For 10-7M concentration, the largest size is 10 times less than that in the 10-5M solution or solid film. For 10-8M concentration, the maximum population is of medium size, but smaller in aggregation number than the minimal population for 10-7M. Aggregation numbers for small, medium, and large populations for 10-9M concentration are the same as for 10-8M, but the population is shifted to smaller aggregation number. Thus, upon dilution, an average aggregation number is gradually shifted to a smaller value. Difference in small, medium, and large particles is observed within 10-7 - 10-8M concentration range.

Paper Details

Date Published: 23 February 2006
PDF: 10 pages
Proc. SPIE 6098, Genetically Engineered Probes for Biomedical Applications, 60980N (23 February 2006); doi: 10.1117/12.650601
Show Author Affiliations
Vadim A. Korolenko, M.V. Lomonosov Moscow State Univ. (Russia)
A.N. Bach Institute of Biochemistry (Russia)
Evgeny Evtushenko, M.V. Lomonosov Moscow State Univ. (Russia)
Alexander L. Rusanov, M.V. Lomonosov Moscow State Univ. (Russia)
A.N. Bach Institute of Biochemistry (Russia)
Nadya Zubova, M.V. Lomonosov Moscow State Univ. (Russia)
A.N. Bach Institute of Biochemistry (Russia)
Ilya N. Kurochkin, M.V. Lomonosov Moscow State Univ. (Russia)
Alexander P. Savitsky, M.V. Lomonosov Moscow State Univ. (Russia)
A.N. Bach Institute of Biochemistry (Russia)


Published in SPIE Proceedings Vol. 6098:
Genetically Engineered Probes for Biomedical Applications
Alexander P. Savitsky; Rebekka M. Wachter, Editor(s)

© SPIE. Terms of Use
Back to Top