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Proceedings Paper

Identification of triplet state and photoisomerization of Cy5 by means of ensemble and single-molecule measurements
Author(s): Zhengxi Huang; Dongmei Ji; Sufan Wang; Andong Xia; Felix Koberling; Matthias Patting; Rainer Erdmann
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Paper Abstract

The triplet state characteristics of Cy5 molecule structurally related to the trans-cis isomerization are studied by means of ensemble and single molecule measurements. Several new deactivations of the singlet state are identified. The delayed fluorescence and phosphorescence from Cy5 molecule were observed. Spectral evidences show that the dim state could originate from the cis-Cy5 with lower excitation rate. The power dependence analysis for the same individual Cy5 molecule indicates an efficient reverse intersystem crossing happened while the T1-Tn absorption is in resonance with the excitation wavelength.

Paper Details

Date Published: 27 February 2006
PDF: 6 pages
Proc. SPIE 6092, Ultrasensitive and Single-Molecule Detection Technologies, 60920Y (27 February 2006); doi: 10.1117/12.650492
Show Author Affiliations
Zhengxi Huang, Institute of Chemistry (China)
Dongmei Ji, Institute of Chemistry (China)
Sufan Wang, Institute of Chemistry (China)
Andong Xia, Institute of Chemistry (China)
Felix Koberling, PicoQuant GmbH (Germany)
Matthias Patting, PicoQuant GmbH (Germany)
Rainer Erdmann, PicoQuant GmbH (Germany)


Published in SPIE Proceedings Vol. 6092:
Ultrasensitive and Single-Molecule Detection Technologies
Jörg Enderlein; Zygmunt K. Gryczynski, Editor(s)

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