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Proceedings Paper

Flying spot en face OCT for monitoring cell distribution in collagen-based constructs
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Paper Abstract

The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.

Paper Details

Date Published: 20 February 2006
PDF: 7 pages
Proc. SPIE 6079, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X, 60790Q (20 February 2006); doi: 10.1117/12.648886
Show Author Affiliations
Konstantinos Kosmidis, Univ. of Liverpool (United Kingdom)
Christopher D. Russell, Medical Laser Institute, Lasers for Life (United Kingdom)
Richard A. Black, Univ. of Liverpool (United Kingdom)
George Dobre, Univ. of Kent (United Kingdom)
Adrian Gh. Podoleanu, Univ. of Kent (United Kingdom)


Published in SPIE Proceedings Vol. 6079:
Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X
Valery V. Tuchin; Joseph A. Izatt; James G. Fujimoto, Editor(s)

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