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Proceedings Paper

Multi-modality imaging of structure and function combining spectral-domain optical coherence and multiphoton microscopy
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Paper Abstract

We have constructed an integrated optical microscope that allows for the simultaneous image acquisition from multiple optical imaging modalities. The microscope consists primarily of hardware for spectral-domain optical coherence microscopy (OCM), multi-photon microscopy (MPM), and second harmonic generation microscopy. The unique configuration of the integrated microscope allows for the acquisition of both anatomical and functional imaging information with particular emphasis in the fields of tissue engineering and tumor biology. By overlaying the structural image obtained from OCM on the functional image obtained simultaneously from MPM (i.e., fluorescent markers imply functional proteins), a more comprehensive view of different tissues can be obtained. In addition, the contemporary analysis of the spectroscopic features enhances contrast in OCM by differentiating different cell and tissue components.

Paper Details

Date Published: 20 February 2006
PDF: 8 pages
Proc. SPIE 6079, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X, 60791D (20 February 2006); doi: 10.1117/12.648745
Show Author Affiliations
Claudio Vinegoni, Beckman Institute/Univ. of Illinois at Urbana-Champaign (United States)
Tyler Ralston, Beckman Institute/Univ. of Illinois at Urbana-Champaign (United States)
Wei Tan, Beckman Institute/Univ. of Illinois at Urbana-Champaign (United States)
Wei Luo, Beckman Institute/Univ. of Illinois at Urbana-Champaign (United States)
Daniel L. Marks, Beckman Institute/Univ. of Illinois at Urbana-Champaign (United States)
Stephen A. Boppart, Beckman Institute/Univ. of Illinois at Urbana-Champaign (United States)


Published in SPIE Proceedings Vol. 6079:
Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X
Valery V. Tuchin; Joseph A. Izatt; James G. Fujimoto, Editor(s)

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