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Proceedings Paper

Scanning total internal reflection fluorescence imaging
Author(s): A. M. Quirke; S. M. Ameer-Beg; M. Parsons; T. Ng; M. Irving; B. Vojnovic
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Paper Abstract

Cell adhesion and focal complex formation require signalling complexes linking cell adhesion molecules to the cytoskeleton. To understand morphogenetic changes associated with tumour cell spreading, migration and tumour cell metastasis, the molecular mechanisms responsible for the regulation, formation and dissolution at the cell-extracellular matrix (ECM) interface need to be identified. In order to achieve this, an improved axial resolution is desirable. We report on the development of a multi-photon (MP) total internal reflection (TIR) fluorescence lifetime imaging (FLIM) system that allows the selective excitation of fluorophores, with such an improved axial resolution. Results from initial experiments are presented. High excitation efficiency is achieved by the use of a Nikon 1.45 NA TIRF objective using annular illumination.

Paper Details

Date Published: 23 February 2006
PDF: 12 pages
Proc. SPIE 6089, Multiphoton Microscopy in the Biomedical Sciences VI, 608907 (23 February 2006); doi: 10.1117/12.648451
Show Author Affiliations
A. M. Quirke, Gray Cancer Institute (United Kingdom)
King's College London (United Kingdom)
S. M. Ameer-Beg, King's College London (United Kingdom)
M. Parsons, King's College London (United Kingdom)
T. Ng, King's College London (United Kingdom)
M. Irving, King's College London (United Kingdom)
B. Vojnovic, Gray Cancer Institute (United Kingdom)


Published in SPIE Proceedings Vol. 6089:
Multiphoton Microscopy in the Biomedical Sciences VI
Ammasi Periasamy; Peter T. C. So, Editor(s)

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